epicentre Q-Beta Replicase QB501250 250 U @ 1 U/μl
产品简介：北京中北林格供应epicentre Q-Beta Replicase QB501250 250 U @ 1 U/μl，中北林格是epicentre总代理，提供epicentre产品货号报价查询。其他包括lucigen总代理，cellscript总代理，Biosearch technologies总代理，epicentre总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。</p>
具体参数
产品名称:Q-Beta Replicase
货号:QB501250
规格:250 U @ 1 U/μl
存储温度:-20℃
品牌：lucigen / epicentre</p>
供应商：中北林格</p>
产地：us</p>
发货地：北京</p>
epicentre Q-Beta Replicase QB501250 250 U @ 1 U/μl 产品说明书
Q-Beta复制酶（QβReplicase）是一种RNA定向的RNA聚合酶，负责复制噬菌体Q-Beta RNA基因组。Blumenthal和Carmichael在一篇评论中介绍了酶的各个方面。1它由四个亚基组成，其中一个由Q-β噬菌体编码，三个由大肠杆菌宿主编码。EPICENTRE的Q-Beta复制酶是从大肠杆菌中纯化的，其中含有表达噬菌体编码亚基的粒。所有四个酶亚基均等比例存在（图1）。
Q-Beta Replicase (Qβ Replicase) is an RNA-directed RNA polymerase that is responsible for replication of the coliphage Q-Beta RNA genome. Various aspects of the enzyme are presented in a review by Blumenthal and Carmichael.1 It is composed of four subunits, one of which is encoded by the Q-Beta phage and three by the E. coli host. EPICENTREs Q-Beta Replicase is purified from E. coli containing a plasmid that expresses the phage-encoded subunit. All four enzyme subunits are present in equal proportions (Fig. 1).
在体外，Q-Beta复制酶可以利用Q-Beta噬菌体RNA以外的其他RNA分子作为模板。这些包括在Q-β复制酶反应中发现的各种亚基因组变异RNA分子，例如中变（MDV）RNA和其他较小的变异RNA。另外，它也可以使用其他“非”模板，例如聚（rC），带有寡核糖核苷酸的RNA或带有多个C残基的RNA。Q-Beta复制酶也可以复制由包埋在中等变数MDV-1 RNA中的RNA序列组成的重组RNA分子。2该酶使用MDV-1 RNA或重组MDV-1 RNA的两条链作为模板，只要酶过，产物的合成就呈指数级。在等温条件下，可以在短时间内实现重组序列的大扩增。
In vitro, Q-Beta Replicase can utilize other RNA molecules besides the Q-Beta phage RNA as templates. These include various subgenomic variant RNA molecules that were found in Q-Beta Replicase reactions, such as midivariant (MDV) RNAs, and other smaller variant RNAs. In addition, it can also use other "non-natural" templates such as poly(rC), RNA primed with an oligoribonucleotide, or RNA tailed with several C residues. Recombinant RNA molecules consisting of RNA sequences embedded within midivariant MDV-1 RNA can also be replicated by Q-Beta Replicase.2 The enzyme uses both strands of MDV-1 RNA or recombinant MDV-1 RNAs as templates, and synthesis of product is exponential so long as the enzyme is in excess. Large amplification of recombinant sequences can be achieved in a short time under isothermal conditions.
单位定义：一个Q-Beta复制酶单位在30°C下使用20 ng / μl的poly（rC）作为模板，在包含33 mM的反应混合物中，在30°C的10分钟内催化将1 nmole的rGTP掺入poly（rG）中。三乙酸酯（pH 7.5），66 mM乙酸钾，10 mM乙酸镁，0.5 mM DTT和1 mM rGTP。
Unit Definition: One unit of Q-Beta Replicase catalyzes the incorporation of 1 nmole of rGTP into poly(rG) in 10 minutes at 30°C using 20 ng/μl of poly(rC) as a template in a reaction mixture containing 33 mM Tris-acetate (pH 7.5), 66 mM potassium acetate, 10 mM magnesium acetate, 0.5 mM DTT, and 1 mM rGTP.
储存缓冲液和条件：含有50 mM Tris-HCl（pH 7.5），100 mM NaCl，1 mM DTT，0.1 mM EDTA和0.1％Triton X-100的50％甘油。储存在-20°C的冰箱中，无霜循环。
Storage Buffer & Conditions: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 0.1% Triton X-100. Store at -20°C in a freezer without a defrost cycle.
10X TA反应缓冲液（另售）： 330 mM三乙酸酯（pH 7.5），660 mM乙酸钾，100 mM乙酸镁和5 mM DTT。
10X TA Reaction Buffer (available separately): 330 mM Tris-acetate (pH 7.5), 660 mM potassium acetate, 100 mM magnesium acetate, and 5 mM DTT.
图1.Qβ复制酶的SDS-PAGE（泳道3）以及大肠杆菌 RNA聚合酶（泳道1）和分子标记（泳道2）。标出了大肠杆菌 RNA聚合酶（β，β，σ和α）和Qβ复制酶（S1，II，Tu和Ts）的亚基名称。
Figure 1. SDS-PAGE of Qβ Replicase (lane 3) along with E. coli RNA polymerase (lane 1) and molecular weight markers (lane 2). The subunit names of E. coli RNA polymerase (β′, β, σ and α) and Qβ Replicase (S1, II, Tu, and Ts) are indicated.
控制：通过SDS聚丙烯酰胺凝胶电泳测定，Q-Beta复制酶的物理纯度大于95％。所有四个亚基均以相等的存在。为了小化Q-Beta模板污染Q-Beta复制酶的可能性，EPICENTER仅使用poly（rC）作为确定活性的模板。不添加模板的Q-Beta复制酶活性的酶促测定无法通过凝胶分析产生可检测到的RNA，因此排除了被酶指数扩增的RNA污染。凝胶分析还表明没有被大肠杆菌 DNA依赖性RNA聚合酶污染。该酶不含可检测的污染性核酸外切酶和核酸内切酶以及RNase活性。
参考
T的Blumenthal和GG的Carmichael。（1979）安。生物化学牧师。 48，525。
Lizardi，PM 等。（1988）生物技术 6，1197。
订购信息 询价请联系 中北林格
Catalog
Q-Beta ReplicaseQB501250250 UnitsEnzyme only. 10X TA Reaction Buffer and NTPs are available separately.
对此产品有兴趣，可联系中北林格获取更多详细信息。</p>
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