TwistDx-TwistAmp exo-货号TAEXO02KIT
TwistAmp exo
(TAEXO02KIT)
产品描述:
TwistAmp® 核扩增产品,能够在15分钟内进行常温下的单分子核检测该技术对硬件设备的要求很低,特别适合用于体外诊断、兽医、食品安全、生物安全、农等领域。TwistAmp exo 用于real-time探检测. 除本组分外, 还含有内切酶3,减少扩增产物的末端拷贝,所以不能用于电泳分析,但在RPA系统中产生更强的动态荧光,便于实时定。
Basic Information
RPA
1) Primers must be 30-35 bases
2) Works best at constant temperature (37-39°C)
3) Amplicons of 80-400bp are preferred
4) TwistAmp® exo Probe required - see overleaf
Set-up (single-plex)1
1) Prepare reaction mix in 1.5ml tube:
Primer A (10μM) 2.1 μl
Primer B (10μM) 2.1 μl
TwistAmp® exo Probe (10μM) 0.6 μl
Rehydration Buffer 29.5 μl
Template and dH2O 13.2 μl
(Total Volume 47.5 μl)
Vortex and spin briefly
2) Add reaction mix to freeze-dried reaction. Pipette to mix.
3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.
4) Place strip in Twista® and start run: 37-39°C, 20 minutes.
4b) For low template copy number, remove strip after 4 minutes, vortex &
spin briefly, replace in Twista®.
WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS
MAGNESIUM IS ADDED.
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