TwistDx-TwistAmp nfo -货号TANFO02KIT
TwistAmp nfo
(TANFO02KIT)
产品描述：
TwistAmp® 核扩增产品，能够在15分钟内进行常温下的单分子核检测该技术对硬件设备的要求很低，特别适合用于体外诊断、兽医、食品安全、生物安全、农等领域。TwistAmp nfo用于末端检测，检测DNA扩增反应. 末端三明治法快速检测，如侧向流试纸法.除本组分之外， nfo核酶，和适合是LF 探 形成新的聚合酶增强底物，无需任何仪器。本品可通过加入exo探作为Amp exo kit的替代物，进行荧光定检测，由于核酶延缓动态反应速度，可用于末端电泳检测。
Basic Information:
RPA
1) Primers must be 30-35 bases
2) Works best at constant temperature (37-39°C)
3) Amplicons of 80-400bp are preferred
4) TwistAmp® LF Probe or TwistAmp® exo Probe 1 required - see overleaf
Set-up (single-plex)2
1) Prepare reaction mix in 1.5ml tube:
Primer A (10μM) 2.1 μl
Primer B (10μM) 2.1 μl
TwistAmp® LF Probe (10μM) 0.6 μl
Rehydration Buffer 29.5 μl
Template and dH2O 13.2 μl
(Total Volume 47.5 μl)
Vortex and spin briefly
2) Add reaction mix to freeze-dried reaction. Pipette to mix.
3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.
4) Incubate at 37-39°C for 20-40 minutes.
4b) For low template copy number,remove strip after 4 minutes, vortex &spin briefly, replace in heating device.
5) For analysis by lateral flow, dilute reacion products 1/50 with PBST and load 10 μl onto the sample pad of appropriate
strip (eg Milenia® HybriDetect strips).
6) Place strip in PBST running buffer.
WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS
MAGNESIUM IS ADDED.
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